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Jul 28 2015

Dental Research Seminar

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Date: July 30, 2015 (Thursday) Time: 12:45 p.m. to 1:45 p.m.
Venue: The Lounge, 7/F, The Prince Philip Dental Hospital

Presenter: Dr. Duangthip Duangporn, PhD Candidate (12:45 p.m. to 1:15 p.m.)
Title: A randomized clinical trial on arresting dentin caries in preschool children by topical fluorides
Abstract: This study was conducted on 371 preschool children with dental caries in primary teeth. At baseline, they were randomly allocated into: Group 1 – annual application of 30% silver diammine fluoride (SDF) solution; Group 2 – three applications of 30% SDF solution at weekly interval; and Group 3 – three applications of 5% sodium fluoride (NaF) varnish at weekly interval. Follow-up examinations every 6 months were conducted to assess whether the treated caries lesions had become arrested. After 24 months, 316 children (85%) remained. For cavitated carious lesions (ICDAS code 5/6), application of SDF solution, either 3 weekly applications at baseline or annual application, arrested active dentin caries faster than 3 weekly applications of NaF varnish at baseline. However, for established carious lesions (ICDAS code 3/4), the three fluoride application protocols were equally efficient in controlling established carious lesions.

Presenter: Ms. Suen Rita Po Chu, MPhil Candidate (1:15 p.m. to 1:45 p.m.)
Title: Dental situation of institutional residents with intellectual disability in Hong Kong
Abstract: Objectives: The aim of this study is to investigate the oral health of intellectually disabled adults living in institutions in Hong Kong. We will also find out their oral health-related behaviours, and difficulties they encounter when accessing dental services.
Methods: The subjects of this study are the intellectually disabled adults who are currently living in government-subvented institutions in Hong Kong. About 750 subjects will be selected from 16 institutions through systematic sampling from the 96 institutions operated by non-governmental organisations, that contributes at least 400 subjects as the final sample size (C.I. = 95%). After obtaining informed consent, care-givers will complete a questionnaire about the dental care behaviours and problems of their cared subjects on their behalf. Additionally, dental information will also be extracted from the patient record of the outreach dental service.

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Jul 16 2015

Dental Research Seminar

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Date: July 23, 2015 (Thursday)
Time: 12:45 p.m. to 1:15 p.m.
Venue: The Lounge, 7/F, The Prince Philip Dental Hospital

Presenter: Mr. Suen Long Kiu Justin, MPhil Candidate

Title: Flavor perception in human brain

Abstract: Flavor perception might be a system far more complicated than we can imagine. For example, we may experience a loss of taste sensation when we block our nose. Indeed, flavor perception is a multisensory integration which may involve taste, smell, tactile, visual and auditory senses. Among different combinations of sensation, the most common one is a combination of taste and smell, which will be mainly focused in this study, stimulated inside or through an oral cavity. The aim of the study is to investigate the human brain response to the interaction between a sour taste and a fruity smell. This presentation will introduce the basic concept of perceiving flavor in neuroscience and some results from the pilot study of functional Magnetic Resonance Imaging.

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Jul 08 2015

Dental Research Seminar

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Date: July 17, 2015 (Friday)
Time: 12:45 p.m. to 1:15 p.m.
Venue: The Lounge, 7/F, The Prince Philip Dental Hospital

Presenter: Mr. Yau Tak Wai, MPhil Candidate

Title: Item Response Models for Child Perception Questionnaire

Abstract: This study aimed to assess factors associated with children’s oral health-related quality of life (OHRQoL) using Child perception Questionnaire (CPQ11-14). The baseline sample consisted of 668 children aged at 12; 436 were followed up 3 years later. Item response theory (IRT) models were used. Some items were found not invariant over 3 years. For the 12-year-old children, heathier oral health behavior, better gum status and some demographic variables were found to be associated with better OHRQoL. With the statistical adjustment of longitudinal invariance, OHRQoL level in general has improved over 3 years but no predictor was found to be associated with OHRQoL in follow‑up. For those with lower family income, their OHRQoL had worsened over 3 years. The use of the IRT models enables the identification of the factors associated with OHRQoL and its changes over time provides important information to oral healthcare researchers and policymakers.

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Jun 03 2015

Dental Research Seminar

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Date: June 9, 2015 (Tuesday) Time: 12:45 p.m. to 1:45 p.m.

Venue: The Lounge, 7/F, The Prince Philip Dental Hospital

 

Presenter: Miss Cao Ying, PhD Candidate (12:45 p.m. to 1:15 p.m.)
Title: Biomimetic remineralisation of hydroxyapatite on human enamel and dentine
Abstract: As the outermost layer of teeth, enamel is susceptible to caries due to the products of bacteria metabolism; or can be lost by trauma or by non-carious tooth loss. As the enamel lesions progress into dentine, tooth hypersensitivity often develops when the exposed dentine is subjected to stimuli. Traditional treatments include replacing the defected tooth structure with dental restorative materials such as amalgam, composite resin, and ceramics. However, marginal leakage, hypersensitivity and secondary caries often happens at the interface. Therefore, there is a need for scientists to design an alternative strategy to repair the tooth lesions. Biomimetic mineralisation is concepts from biomineralisation trying to transfer biomineralisation principles to the regeneration of new biomaterials. This study aimed to remineralise the demineralised enamel and dentine using biomimetic mineralisation methods.

 

Presenter: Miss Ito Leticia, PhD Candidate (1:15 p.m. to 1:45 p.m.)
Title: Effects of silver diamine fluoride on dentine demineralization and in combined use with laser irradiation
Abstract: In the mouth, there is always a dynamic process of demineralisation and remineralisation of the exposed teeth. Caries develops when the balance tips to demineralisation; but it can be arrested when the lesion is overwhelmed by remineralisation. Results from clinical trials showed that silver diamine fluoride can arrest and reharden carious lesions. Investigation also showed that enamel and dentine are harder and less soluble after application of SDF. In vitro studies also found that SDF can prevent Actinomyces naeslundii and Streptococcus mutans biofilm formation and reduce further demineralization. Studies also suggest laser irradiation after topical sodium fluoride application increase fluoride uptake on dental hard tissues. Current literature however found no study reporting the effect of laser irradiation on dentine treated with SDF under bacteria challenge. Therefore, this study investigated the effects of SDF on dentine demineralization and in combined use with laser irradiation under cariogenic biofilm.

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May 20 2015

Dental Research Seminar

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Date: May 27, 2015 (Wednesday)
Time: 12:45 p.m. to 1:45 p.m.
Venue: The Lounge, 7/F, The Prince Philip Dental Hospital

Presenter: Ms. Basudan Aishah Mohammed A, PhD Candidate (12:45 p.m. to 1:15 p.m.)

Title: ZONE-SPECIFIC GENE EXPRESSION OF MANDIBULAR CONDYLAR CARTILAGE: A Promising Approach or an Overcomplicated Strategy?

Abstract: Mandibular condylar cartilage (MCC) possesses a unique multizonal structure to perform its function as a lubricating and load-bearing surface in the TMJ and as a growth plate cartilage. Trauma and/or pathogenesis of the MCC, which has limited capacity for regeneration, are significant causes of morbidity in human beings. Thus, tissue engineering may provide an ideal solution, but current zone-specific information is limited to a small set of genes and proteins. Resolving functional and phenotypic differences between cell populations of the MCC is important to understand mechanisms of homeostasis and pathogenesis. In addition, the identification of zone specific differential gene expression is useful in tissue engineering approaches to recapitulate the native zonal architecture.

The aim of our study is to obtain new insights into the characterization of MCC cells by investigating gene expression profiles of individually isolated MCC zones. The question that arises: is this zonal-based investigation a promising approach or just an overcomplicated strategy?

Presenter: Miss Ren Chong, PhD Candidate (1:15 p.m. to 1:45 p.m.)

Title: A randomized controlled trial of a laser-aided orthodontic treatment for patients with periodontitis

Abstract: The objective of this double-blind randomized controlled trial is to assess the effects of low-level laser therapy (LLLT) in orthodontic patients with periodontitis on pain, periodontal inflammation, alveolar bone remodeling and dentin sensitivity. Thirty-five nonsmoking patients (male or female, 18-60 years old) with controlled chronic periodontitis will be recruited. After insertion of the first archwire, one side of the arch will be randomly assigned to repeated LLLT (940nm, continuous mode and energy density: 3.57 J/cm2) and the other side to placebo laser. Following parameters will be recorded on selected time-points during the 12-month follow-up: visual analogue scale, bite force, cytokines in gingival crevicular fluid, bacteria levels in supragingival plaque, clinical parameters and cone-beam computed tomography. This study will provide first-hand evidence for the incorporation of LLLT in adjunctive orthodontic treatment for patients with chronic periodontitis.

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Apr 28 2015

Dental Research Seminar

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Date: May 5, 2015 (Tuesday)
Time: 12:45 p.m. to 1:45 p.m.
Venue: The Lounge, 7/F, The Prince Philip Dental Hospital

Presenter: Dr. Bhoj Manasi, PhD Candidate (12:45 p.m. to 1:15 p.m.)

Title: A lab-centered approach for initiating early regeneration of dental pulp using specialized alginate biomimetic microenvironments.

Abstract: There is a significant need in modern endodontics to have living replacement for dental pulp that can salvage the infected necrotic tooth. The first step towards fabricating a dental pulp tissue is to construct a suitable cellular structural microenvironment. In this study we establish an environment by judicious selection of hDPSC and HUVEC encapsulated in RGD alginate infused with physiologically relevant quantities of VEGF and FGF. These environment constructs were fabricated in template of gutta percha shape mold. They were analyzed for viability, proliferation, morphological changes, distribution and growth factor release kinetics. Results from confocal and SEM showed uniform distribution of cells along the length of construct. They were viable with significant rate of proliferation, morphological changes and cell-clustering phenomena noted with formulation in constructs having one or both added growth factors than in growth factor-free constructs. The release kinetics showed significant results in groups with dual growth factors. We conclude that combining VEGF and FGF in 1:3 ratio to co-culture of DPSC-HUVEC could initiate denovo regeneration.

Presenter: Dr. Zakir Muhammad, PhD Candidate (1:15 p.m. to 1:45 p.m.)

Title: Bonding dissimilar materials in dentistry

Abstract: Bonding of dental restorative materials to tooth tissues is one of the most important aspects in dentistry. To meet the harsh oral environment coupling agents are used to enhance the adhesion between different materials. There has been considerable research on silane coupling agents compared to other coupling agents. Adhesion of metals and resin cements is the most important aspect for the longevity of the prosthesis. Titanium is used as a material of choice for dental implants and other metal dental prosthesis. Our aim is to use an optimized novel silane system and compare and contrast its adhesive properties to the commercially available surface modifiers and resin cements when titanium and resin cements are bonded. Different mechanical tests such as shear bond adhesion test, fractural analysis, scanning electron microscopy, atomic force microscopy (for surface roughness), energy dispersive X-ray spectroscopy are some tests which are going to be conducted. All the tests are going to be done after the specimens have been aged in water and after thermocycling. All the obtained data will be statistically analyzed and presented in the thesis and published studies.

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Apr 27 2015

Dental Research Seminar

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Date: April 30, 2015 (Thursday)
Time: 12:45 p.m. to 1:15 p.m.
Venue: The Lounge, 7/F, The Prince Philip Dental Hospital

Presenter: Dr. Ab Malik Normaliza Binti, PhD Candidate

Title: Oral Health Promotion Intervention Among Stroke-Carers and Patients

Abstract: Disability caused by stroke can decrease patients’ quality of life through impacts on daily life activities. Rehabilitation services for patients with stroke are dedicated to improve the condition of patients and to support independence through education and training.  Oro-motor function and clearance are commonly perturbed following stroke, and daily toothbrushing is also impaired due to reduced manual dexterity.  Dental plaque accumulates at a faster rate, and opportunistic bacteria and yeast proliferate. While maintenance of oral health is a great challenge for patients after stroke, health care staff and caregivers are ideally positioned to support and assist these patients to maintain oral hygiene so as to prevent not only oral diseases but also life-threatening infections such as aspiration pneumonia.  Evaluations of oral care provided by caregivers and approaches for optimizing such care have received little attention.  Thus, this study aims to evaluate the factors that determine the oral health behavior of stroke care providers, and the impact of a tailored Computer-Assisted-Learning (CAL) programme.

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Apr 22 2015

Dental Research Seminar

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Date: April 28, 2015 (Tuesday) Time: 12:45 p.m. to 1:45 p.m.
Venue: The Lounge, 7/F, The Prince Philip Dental Hospital

Presenter: Miss Li Xin, PhD Candidate (12:45 p.m. to 1:15 p.m.)
Title: Bioimimetic Nature-Derived Antibacterial Surfaces for Modified Denture and Dental Restorative Materials

Abstract: Control of the oral microbiome under different disease, inflammatory and degenerative challenges is possible with antibiotics, anti-quorum sensing molecules and toxic heavy metals such as, silver nanoparticles. However, bacteria can quite rapidly evolve resistance to chemical agents. They cannot evolve mutations to counter physical structures that are able to damage and destroy them. Therefore, attention has focused on the use of micro- and nanostructures to kill pathogenic bacteria particularly derived from nature where self-cleaning surfaces are common.
In dentistry the ultimate aim is to develop strategies that affect species that have become virulently pathogenic and restore a normal interdependent balanced microbiome. Previously it has been reported that Cicada wing surfaces can selectively remove pathogenic, gram negative while maintaining non-pathogenic species. The post-like protrusions are effective in rupturing the membrane of thin-walled bacteria represented by the gram negative staining classification. We have studied a range of potential nature-derived candidates as templates for antibacterial surface structure. Gecko skin fulfils criteria for a superior antibacterial surface structure, because it consists of high aspect ratio nanohairs, confined in tight spacing and eliciting superhydrophobicity. Such intricate structures can be copied in any material allowing for their exploitation in biomedicine and dentistry.
The aim of this study is to measure the short-term bactericidal properties of gecko skin, and Epoxy resin polymer imprints and assess their feasibility to control two ubiquitous species of “surface associating” pathogenic oral bacteria with virulent biofilm forming potential. The goal is to confirm whether these surface structures can be usefully applied as bactericidal coatings at the surface of dental implants and restorations requiring bacterial control management.

 

Presenter: Dr. Govindool Sharaschandra Reddy, PhD Candidate (1:15 p.m. to 1:45 p.m.)
Title: Possibility of Key-Hole Revision of Endodontic Treatment

Abstract: The nonsurgical revision endodontic treatment may involve the perforation of intact crown, decementation of crown or crown and bridges and disobturation of radiographically satisfactory obturation, which may be unacceptable to the patient many a times. The option of surgical approach may not appeal to the patient or it may be too invasive in terms of the size of the persistent lesion or new lesion formed or just symptomatic tooth as described by the patient all of which have known to be of microbiological origin.
This research evaluates the possibility of an approach to a surgical technique, which is based on the concept of keyhole surgery in conjunction with photodynamic dye and laser activation to eradicate the extra-radicular infection in endodontically treated tooth and the periapical region. The feasibility is assessed in a series of experiments to evaluate the parameters that make this procedure a clinical possibility

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Apr 15 2015

Dental Research Seminar

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Date: April 22, 2015 (Wednesday)
Time: 12:45 p.m. to 1:45 p.m.
Venue: The Lounge, 7/F, The Prince Philip Dental Hospital

Presenter: Dr. Du Yanlin, PhD Candidate

Title: Improving Oral Health for Children with Autism Spectrum Disorders

Abstract: Autism Spectrum Disorders (ASD) is a spectrum of developmental disorders characterized by impairments in communication and social skills. Maintaining good oral health requiring special care is a challenge because of multiple learning, communication and cogitative impairments. This has been the focus of my thesis with a theme of research relating to special care dentistry.

A systematic review of the oral health status of individuals with autism spectrum disorders was undertaken across five electronic databases employing PRISMA guidelines. The initial search yielded 727 papers, among which 36 were identified as potentially effective papers and 11 ‘effective’ case-control studies informed this review. Variations in oral health existed among children with and without ASD in terms of dental caries experience, periodontal health, malocclusion, dental injuries and bruxism. This has implication for special care practice and policies.

To understand ASD and oral health in the Hong Kong context a population-wide oral epidemiological study was undertaken among 17 randomly selected registered Special Child Care Centres. An age (±6 months) and gender matched sample of children from mainstreams preschools were recruited as the ‘control group’. Comprehensive oral health assessments were undertaken for over 500 children. Difference in clinical oral health status existed among preschool children with and without ASD in terms of dental caries experience (dmft>0), periodontal health (pp<0.001).

A clinical trial was undertaken to evaluate the effectiveness of a tooth-brushing visual pedagogy to improve oral health and enhance oral self-care among children with ASD in both intervention and control groups. Improvements in oral hygiene status and gingival health were observed over the 6-month period. Regression analyses identified the visual pedagogy intention was a significant factor associated with improved oral health (p This has implications for safeguarding and promoting good oral health among children with ASD in Hong Kong and beyond.

 

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Feb 05 2015

Dental Research Seminar

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Date: February 11, 2015 (Wednesday)

Time: 12:45 p.m. to 1:15 p.m.

Venue: The Lounge, 7/F, The Prince Philip Dental Hospital

Presenter: Miss Yang Xinchen, PhD Candidate

Title: Multidimensional assessments on outreach dental services for older persons using long-term care services in Hong Kong

Abstract: Older persons using long-term care (LTC) services in Hong Kong are, in general, frail and suffering from various kinds of physical and/or mental impairment. Due to the frailty, a lower ability to take care of themselves and irregular dental checkup habit, the LTC users have unsatisfactory oral health status. Outreach dental service teams (ODT) program for the older persons is one of the major projects subsidized by the Hong Kong Government. This program targets at improving oral health of institutionalized older persons and older persons using day care centers/units through oral health education and provision of on-site dental treatments.

The aims of this study are to investigate the achievements and deficiencies of the ODT program from 3 dimensions: the recipients, the providers and the administrators of this outreach dental service.  Both quantitative and qualitative methods will be employed.

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